Related Experiment Video
Updated: Jul 8, 2026

Eukaryotic Polyribosome Profile Analysis
Published on: June 15, 2010
Transcript-specific translational regulation in the unfolded protein response of Saccharomyces cerevisiae
Tom Payne1, Colin Hanfrey, Amy L Bishop
1School of Biology, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
Abstract:
Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes stress and induces the unfolded protein response (UPR). Genome-wide analysis of translational regulation in response to the UPR-inducing agent dithiothreitol in Saccharomyces cerevisiae is reported. Microarray analysis, confirmed using qRT-PCR, identified transcript-specific translational regulation. Transcripts with functions in ribosomal biogenesis and assembly were translationally repressed. In contrast, mRNAs from known UPR genes, encoding the UPR transcription factor Hac1p, the ER-oxidoreductase Ero1p and the ER-associated protein degradation (ERAD) protein Der1p, were enriched in polysomal fractions, indicating translational up-regulation. Splicing of HAC1 mRNA is shown to be required for efficient ribosomal loading.
More Related Videos
14:29Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells
Published on: December 25, 2021
09:21Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
Published on: October 22, 2018
Related Concept Videos
Translational Regulation
Regulation of the Unfolded Protein Response
Regulation of Expression at Multiple Steps
The Unfolded Protein Response
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcriptional Regulation: Riboswitches