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Related Experiment Video

Updated: Jul 8, 2026

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

Published on: August 19, 2021

Exploiting glycogen synthase kinase 3beta flexibility in molecular recognition.

Michael P Mazanetz¹, Ian M Withers, Charles A Laughton

¹Centre for Biomolecular Sciences and School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, U.K.

Biochemical Society Transactions

|January 23, 2008

Summary

Glycogen synthase kinase 3beta (GSK3beta) plays a role in various diseases. Understanding its dynamic nature and protein plasticity is key for developing new drugs, as shown by the active-site pressurization (ASP) method.

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Last Updated: Jul 8, 2026

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

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Modeling an Enzyme Active Site using Molecular Visualization Freeware

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Published on: December 25, 2021

Area of Science:

Biochemistry
Pharmacology
Structural Biology

Background:

Glycogen synthase kinase 3beta (GSK3beta) regulates critical cellular pathways through protein phosphorylation.
Dysregulation of GSK3beta is implicated in diseases like diabetes and Alzheimer's.
Targeting GSK3beta offers therapeutic potential, necessitating a deep understanding of its active site.

Purpose of the Study:

To investigate the dynamic characteristics of GSK3beta.
To emphasize the significance of protein plasticity in structure-based drug design.
To introduce and exemplify the active-site pressurization (ASP) method.

Main Methods:

Computational analysis of GSK3beta dynamics.
Application of the active-site pressurization (ASP) method.
Structure-based analysis of enzyme-inhibitor interactions.

Main Results:

GSK3beta exhibits significant protein plasticity.
The ASP method effectively probes active site dynamics.
Understanding these dynamics is crucial for rational drug design.

Conclusions:

Protein plasticity is a critical factor in GSK3beta inhibitor recognition.
The ASP method provides valuable insights for structure-based drug design strategies.
Targeting GSK3beta's dynamic nature can lead to novel therapeutic interventions.