Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
Nuclear Export01:42

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic Accuracy of CBCT Versus 2D Radiography for Various Dental Pathologies: A Systematic Review and Meta-Analysis.

Australian endodontic journal : the journal of the Australian Society of Endodontology Inc·2026
Same author

Genetic and Pathogenic Differentiation of <i>Fusarium oxysporum</i> Isolates from Ginger.

Journal of fungi (Basel, Switzerland)·2026
Same author

Polyphenols in Cancer Therapy: Recent Advances, Mechanistic Pathways, and Therapeutic Insights.

Current pharmaceutical design·2026
Same author

A chromosome-level genome assembly of <i>Rhizopus stolonifer</i> associated with passion fruit flower rot.

Frontiers in microbiology·2026
Same author

Rational Design and Evaluation of Novel TGR5 Agonists for Diabetes.

Molecules (Basel, Switzerland)·2026
Same author

Decoding the Anticancer Potential of Natural N-Heterocycles: Molecular Frameworks, Bioactivity, and Mechanistic Advances.

Anti-cancer agents in medicinal chemistry·2026

Related Experiment Video

Updated: Jul 8, 2026

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
11:19

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Published on: November 17, 2019

Towards defining the nuclear proteome.

J Lynn Fink1, Seetha Karunaratne, Amit Mittal

  • 1ARC Centre of Excellence in Bioinformatics, The University of Queensland, St Lucia, Queensland, 4072, Australia.

Genome Biology
|January 24, 2008
PubMed
Summary

Researchers identified 2,568 mammalian nuclear proteins, revealing the nuclear proteome comprises at least 14% of the entire proteome. This dataset aids in evaluating computational methods for nuclear protein identification.

More Related Videos

Quantitative Analysis of Chromatin Proteomes in Disease
08:11

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

A Versatile Pipeline for Analyzing Dynamic Changes in Nuclear Bodies in a Variety of Cell Types
06:33

A Versatile Pipeline for Analyzing Dynamic Changes in Nuclear Bodies in a Variety of Cell Types

Published on: June 28, 2024

Related Experiment Videos

Last Updated: Jul 8, 2026

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
11:19

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Published on: November 17, 2019

Quantitative Analysis of Chromatin Proteomes in Disease
08:11

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

A Versatile Pipeline for Analyzing Dynamic Changes in Nuclear Bodies in a Variety of Cell Types
06:33

A Versatile Pipeline for Analyzing Dynamic Changes in Nuclear Bodies in a Variety of Cell Types

Published on: June 28, 2024

Area of Science:

  • Cell Biology
  • Proteomics

Background:

  • The cell nucleus is a complex organelle.
  • Accurate definition of nuclear protein content is crucial for systematic characterization.

Purpose of the Study:

  • To experimentally define the mammalian nuclear proteome.
  • To establish a high-quality dataset for evaluating computational methods.

Main Methods:

  • High-throughput subcellular localization protocol for full-length proteins.
  • Literature review for experimentally validated nuclear proteins.

Main Results:

  • Direct evidence for 2,568 mammalian nuclear proteins identified.
  • Nuclear proteome constitutes at least 14% of the entire proteome.
  • Dataset used to evaluate computational approaches for nuclear protein identification.

Conclusions:

  • Experimental validation confirms the nuclear proteome's substantial size (≥14% of the proteome).
  • The generated dataset serves as a benchmark for computational prediction methods.
  • Researchers can use this data to refine criteria for inferring nuclear proteome size and composition.