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Towards defining the nuclear proteome.

J Lynn Fink1, Seetha Karunaratne, Amit Mittal

  • 1ARC Centre of Excellence in Bioinformatics, The University of Queensland, St Lucia, Queensland, 4072, Australia.

Genome Biology
|January 24, 2008
PubMed
Summary
This summary is machine-generated.

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Researchers identified 2,568 mammalian nuclear proteins, revealing the nuclear proteome comprises at least 14% of the entire proteome. This dataset aids in evaluating computational methods for nuclear protein identification.

Area of Science:

  • Cell Biology
  • Proteomics

Background:

  • The cell nucleus is a complex organelle.
  • Accurate definition of nuclear protein content is crucial for systematic characterization.

Purpose of the Study:

  • To experimentally define the mammalian nuclear proteome.
  • To establish a high-quality dataset for evaluating computational methods.

Main Methods:

  • High-throughput subcellular localization protocol for full-length proteins.
  • Literature review for experimentally validated nuclear proteins.

Main Results:

  • Direct evidence for 2,568 mammalian nuclear proteins identified.
  • Nuclear proteome constitutes at least 14% of the entire proteome.
  • Dataset used to evaluate computational approaches for nuclear protein identification.

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Conclusions:

  • Experimental validation confirms the nuclear proteome's substantial size (≥14% of the proteome).
  • The generated dataset serves as a benchmark for computational prediction methods.
  • Researchers can use this data to refine criteria for inferring nuclear proteome size and composition.