Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Renin substrate in granules from rat kidney cortex.

B J Morris, C I Johnston

The Biochemical Journal

|March 15, 1976

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Popliteomeniscal fascicles tears with lateral meniscus instability: outcomes of arthroscopic surgical technique at mid-term follow-up.

Archives of orthopaedic and trauma surgery·2022

Same author

Comparable clinical and functional outcomes after anterior cruciate ligament reconstruction over and under 40 years of age.

Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA·2019

Same author

No relationship between critical shoulder angle and glenoid erosion after shoulder hemiarthroplasty: a comparative radiographic study.

Archives of orthopaedic and trauma surgery·2017

Same author

A novel broth medium for enhanced growth of Francisella tularensis.

Letters in applied microbiology·2017

Same author

Mice haploinsufficient for Map2k7, a gene involved in neurodevelopment and risk for schizophrenia, show impaired attention, a vigilance decrement deficit and unstable cognitive processing in an attentional task: impact of minocycline.

Psychopharmacology·2016

Same author

Altered functional brain network connectivity and glutamate system function in transgenic mice expressing truncated Disrupted-in-Schizophrenia 1.

Translational psychiatry·2015

Renin substrate is present within rat kidney cells, localized to specific particles. This finding supports a proposed mechanism for intracellular angiotensin production in the kidney.

Area of Science:

Biochemistry
Cell Biology
Renal Physiology

Background:

The renin-angiotensin system plays a crucial role in blood pressure regulation.
Understanding the intracellular localization and function of renin substrate is essential for elucidating local angiotensin production.

Purpose of the Study:

To investigate the subcellular localization of renin substrate within rat kidney cortex cells.
To characterize the biochemical properties of renin substrate-containing particles.
To propose a mechanism for intrarenal angiotensin production.

Main Methods:

Subcellular fractionation of rat kidney cortex.
Incubation with rat renin to generate angiotensin I.
Differential and isopycnic gradient centrifugation.

Related Experiment Videos

Molecular weight determination.

Main Results:

Renin substrate was detected within intracellular fractions of rat kidney cortex.
Highest concentrations of renin substrate were found in particulate fractions with specific sedimentation and density characteristics.
Similar molecular weights of renin substrate were observed in kidney, liver, and plasma.

Conclusions:

Renin substrate is present intracellularly in the renal cortex, associated with specific subcellular particles.
These findings support a model for intrarenal angiotensin production via a subcellular reaction pathway.