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Slits and Robos in the developing chicken inner ear.

Andrea C Battisti¹, Donna M Fekete

¹Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47906-2054, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|January 24, 2008

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Slit and Robo gene expression was studied in the developing chick inner ear. These molecules may guide otic neuroblasts and axons, and maintain sensory/nonsensory boundaries.

Area of Science:

Neuroscience
Developmental Biology
Genetics

Background:

Mechanosensory hair cells in the chick inner ear synapse onto afferent neurons of the statoacoustic ganglion (SAG).
During development, these neurons extend a central process to the brain and a peripheral process into one of eight sensory organs.
A combination of cues, including chemoattractants and chemorepellents, direct otic axons to their peripheral targets.

Purpose of the Study:

To examine the expression of Slit and Robo gene transcripts in the developing chick inner ear.
To evaluate the role of Slit/Robo signaling in otic axon guidance and the maintenance of sensory/nonsensory boundaries.

Main Methods:

Expression analysis of Slit and Robo transcripts in the chick inner ear.
Utilized Hamburger and Hamilton stages 14-37 (embryonic day 2-11).

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Main Results:

Robo2 and slit2 transcripts were detected in migrating neuroblasts and the statoacoustic ganglion (SAG).
Both Slit and Robo transcripts were found in the otic epithelium.
Expression data suggests a role in repulsion of otic neuroblasts and axons.

Conclusions:

Slit/Robo signaling may be involved in the initial guidance and repulsion of otic neuroblasts and axons.
Expression patterns suggest a novel role for Slit/Robo in maintaining sensory/nonsensory boundaries within the inner ear.