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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jul 8, 2026

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
14:45

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Published on: August 6, 2014

Transgenic lambda medaka as a new model for germ cell mutagenesis.

Richard N Winn1, Audrey J Majeske, Charles H Jagoe

  • 1Aquatic Biotechnology and Environmental Laboratory, Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia. rwinn@uga.edu

Environmental and Molecular Mutagenesis
|January 24, 2008
PubMed
Summary

We developed a new lambda transgenic medaka fish model to study mutations in germ cells passed to offspring after exposure to ethyl-nitrosourea (ENU). This model efficiently detects mutations and reveals differences based on germ cell stage, aiding genetic risk assessment.

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Last Updated: Jul 8, 2026

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
14:45

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Published on: August 6, 2014

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
11:06

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Published on: February 24, 2014

The Lambda Select cII Mutation Detection System
07:08

The Lambda Select cII Mutation Detection System

Published on: April 26, 2018

Area of Science:

  • Genetics
  • Toxicology
  • Developmental Biology

Background:

  • Studying heritable mutations from mutagen exposure requires sensitive models.
  • Germ cell mutagenesis is crucial for understanding genetic damage transmission to progeny.

Purpose of the Study:

  • To evaluate lambda transgenic medaka as a novel model for germ cell mutagenesis.
  • To analyze mutation frequencies, spectra, and patterns in offspring from mutagen-exposed parents.

Main Methods:

  • Utilized lambda transgenic medaka carrying the cII mutation target gene.
  • Exposed male medaka to ethyl-nitrosourea (ENU) at different germ cell stages.
  • Detected and quantified cII mutations in progeny.

Main Results:

  • Successfully detected mutations in the cII gene in offspring.
  • Observed higher mutation frequencies from postmeiotic germ cells (11%) compared to premeiotic cells (3.5%).
  • Identified mosaic and whole-body mutants, with mosaic mutants predominating from postmeiotic and stem cells; noted delayed mutations.

Conclusions:

  • Lambda transgenic medaka is an efficient and sensitive model for assessing germ cell mutations.
  • Germ cells play a role in post-fertilization genomic instability.
  • This model enhances understanding of mutagenesis mechanisms and genetic health risk assessment.