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Colorimetric assay for sensitive poly(styrene sulfonate) quantification in a template directed

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  • 1RWTH Aachen University, Department of Biotechnology, Aachen, Germany.

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Area of Science:

  • Biotechnology
  • Materials Science
  • Analytical Chemistry

Background:

  • Layer-by-layer (LbL) technology is a versatile tool for bioencapsulation, offering control over wall properties, thickness, and permeability.
  • Quantifying polyelectrolyte deposition, specifically poly(allylamine) hydrochloride (PAH) and poly(styrene sulfonate) sodium salt (PSS), on surfaces typically requires expensive and sophisticated equipment.
  • Accurate monitoring of PSS deposition is crucial for successful LbL coating and bioencapsulation.

Purpose of the Study:

  • To develop a simple, cost-effective, and accurate method for quantifying PSS during LbL coating.
  • To provide an alternative to existing expensive quantification techniques for LbL processes.
  • To facilitate broader application of LbL technology in biotechnology through improved process control.

Main Methods:

  • A colorimetric assay utilizing the Bradford reagent was developed for PSS quantification.
  • The method relies on the spectral shift of the Bradford reagent's absorption maximum from 465 to 680 nm upon binding to PSS.
  • Standard laboratory equipment and commercially available reagents were employed.

Main Results:

  • The Bradford reagent assay accurately quantifies submicrogram amounts of dissolved PSS.
  • The method demonstrates significant accuracy and excellent reproducibility in spectral quantification.
  • Successful quantification of polyelectrolyte loadings was achieved on various LbL template particles.

Conclusions:

  • The developed colorimetric assay offers a practical and affordable alternative for monitoring PSS in LbL processes.
  • This method can be adopted as a standard laboratory technique for controlling LbL encapsulation.
  • The enhanced control and accessibility will significantly broaden the applicability of LbL technology in biotechnology.