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Updated: Jul 8, 2026
Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium
Published on: July 14, 2020
Photopic electroretinograms of mGluR6-deficient mice
Toshiyuki Koyasu1, Mineo Kondo, Kentaro Miyata
1Department of Ophthalmology, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya, Japan.
Purpose:
To study the properties of the photopic electroretinograms (ERGs) of the metabotropic glutamate receptor subtype 6 (mGluR6)-deficient mice and to investigate the contribution of cone ON-and OFF-pathways to the mouse photopic ERGs.
Methods:
Photopic ERGs were recorded from mGluR6-deficient and wild-type mice. Photopic ERGs were also recorded after an intravitreous injection of cis-2,3 piperidine dicarboxylic acid (PDA) to block the transmission of signals from the photoreceptors to the OFF-bipolar cells, horizontal cells, and other inner retinal neurons.
Results:
The amplitude of the b-wave of the photopic ERG was severely reduced in mGluR6-deficient mice, but a small, slow, positive component was seen after the a-wave. Intravitreous injection of PDA eliminated this positive component.
Conclusions:
The mGluR6-deficient mouse is a useful animal model to study the contribution of the ON-and OFF-pathways to the mouse ERG.
