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Updated: Jul 8, 2026

Determination of the Gas-phase Acidities of Oligopeptides
Published on: June 24, 2013
An electrospray mass spectrometric method for accurate mass determination of highly acid-sensitive phosphoramidites
Zoltán Kupihár1, Zoltán Timár, Zsuzsanna Darula
1Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO, USA. kupi@ovrisc.mdche.u-szeged.hu
Abstract:
An accurate mass determination method utilizing electrospray ionization mass spectrometry is described for analysis of several different types of phosphoramidites that are extremely acid-sensitive compounds. An earlier method, which applied a LiCl/acetonitrile system, was extended for this special application by using polymeric standards including poly(ethylene glycol) (PEG), poly(ethylene glycol) dimethyl ether (PDE) and poly(propylene glycol) (PPG). Concentrations of standards, samples and LiCl were optimized and potential impurities that affect the analyses were also investigated.
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