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Updated: Jan 24, 2026

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
Published on: April 28, 2022
Label-free coherent anti-stokes Raman scattering imaging of coexisting lipid domains in single bilayers
1Weldon School of Biomedical Engineering and Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA. li7@purdue.edu
Abstract:
Laser-scanning coherent anti-Stokes Raman scattering (CARS) microscopy was used to image lipid domains in single bilayers without any labeling. On the basis of the molecular packing density difference between liquid-disordered (Ld), liquid-ordered (Lo), and gel (So) phases, clear vibrational contrasts were generated between coexisting domains in a single bilayer of DOPC/DPPC (1:1) and DOPC/DPPC/cholesterol (4:4:2). The method reported here can be potentially applied to study phase segregation in live cell membranes which are highly heterogeneous and dynamic.
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