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[Time effect of dentin matrix protein 1 and osteoclast expression during mandibular fracture healing in rats]
Tie-xia Hu1, Zu-bing Li, Zhi Li
1Department of Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China.
Objective:
To study the expression of dentin matrix protein 1 (DMP1) and osteoclast in callus at different healing period of mandibular fracture in a adult Wistar rat model.
Methods:
The mandibular fracture model of Wistar rats at the left mandibular ramus was established. The callus in the fractured site and the normal mandible were amputated at the 5th, 7th, 14th and 21st day after the fracture. HE staining was used to observe the condition of fracture healing and TRAP staining used to observe the activation of osteoclast. The expression of DMP1 was detected in the callus by using immunohistochemical method.
Results:
The number of osteoclasts reached a peak from the 14th to 21st day. The expression of DMP1 became very active from the 7th to 14th day.
Conclusions:
The expression of DMP1 and osteoclast during fracture healing exhibited time effect.
