Related Experiment Video
Updated: Jul 8, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
p21-LacZ reporter mice reflect p53-dependent toxic insult
Douglas B Vasey1, C Roland Wolf, Thomas MacArtney
1Division of Gene Function and Development, Roslin Institute, Roslin, Midlothian, EH25 9PS, UK. douglas.vasey@bbsrc.ac.uk
Abstract:
There is an urgent need to discover less toxic and more selective drugs to treat disease. The use of transgenic mice that report on toxic insult-induced transcription can provide a valuable tool in this regard. To exemplify this strategy, we have generated transgenic mice carrying a p21-LacZ transgene. Transgene activity reflected endogenous p21 gene activation in various tissues, displayed compound-specific spatial expression signatures in the brain and immune tissues and enabled p53-dependent and p53-independent responses to be identified. We discuss the application of these mice in delineating the molecular events in normal cellular growth and disease and for the evaluation of drug toxicity.
More Related Videos
Related Concept Videos
Abnormal Proliferation
Mouse Models of Cancer Study
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

