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Microfluidics in Assessing Platelet Function
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Microfluidics in Assessing Platelet Function

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Assessment of ADP-induced platelet aggregation with light transmission aggregometry and multiple electrode platelet

Dirk Sibbing1, Siegmund Braun, Stefan Jawansky

  • 1Deutsches Herzzentrum München and 1. Medizinische Klinik rechts der Isar, Technische Universität München, Munich, Germany. dirk@sibbing.net

Thrombosis and Haemostasis
|January 25, 2008
PubMed

Insights

Multiple electrode platelet aggregometry (MEA) offers a rapid and standardized method for assessing platelet function after clopidogrel treatment. This new approach correlates well with traditional light transmission aggregometry (LTA) for evaluating antiplatelet response.

Area of Science:

  • Cardiovascular Medicine
  • Hematology
  • Clinical Chemistry

Background:

  • Platelet aggregation measurement using light transmission aggregometry (LTA) in platelet-rich plasma (PRP) predicts outcomes post-percutaneous coronary intervention (PCI).
  • LTA is time-consuming and lacks standardization, creating a need for faster, more reliable methods to assess platelet function, especially after antiplatelet therapy like clopidogrel.

Purpose of the Study:

  • To evaluate the efficacy of multiple electrode platelet aggregometry (MEA) in assessing platelet function.
  • To compare MEA with LTA in measuring adenosine diphosphate (ADP)-induced platelet aggregation before and after clopidogrel administration in patients undergoing coronary angiography.

Main Methods:

  • ADP-induced platelet aggregation was measured using both LTA (in PRP) and MEA (in whole blood) on the Multiplate analyzer.
  • Blood samples were collected from 149 patients post-clopidogrel treatment and from 60 patients pre- and post-treatment.

Main Results:

  • Platelet aggregation significantly decreased after clopidogrel administration when measured by MEA (P < 0.0001).
  • MEA measurements of ADP-induced platelet aggregation showed a significant correlation with LTA results (Spearman's rho = 0.71; P < 0.0001).

Conclusions:

  • MEA is a fast and standardized method for assessing platelet response to ADP.
  • MEA provides reliable assessment of platelet function and antiplatelet effects, correlating well with LTA, making it valuable for clinical practice.

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