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Updated: Jul 8, 2026

Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Fluorescent nanoparticle for bacteria and DNA detection.

Wenjun Zhao¹, Lin Wang, Weihong Tan

¹Department of Chemistry, Center for Research at Bio/Nano Interface, Shands Cancer Center and UF Genetics Institute, University of Florida, Gainesville 32611-7200, USA.

Advances in Experimental Medicine and Biology

|January 26, 2008

Summary

Researchers developed a rapid bioassay using dye-doped silica nanoparticles for detecting single bacterial cells and DNA. This nanotechnology offers ultrasensitive detection for medical and biotechnological applications.

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Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

Area of Science:

Bionanotechnology
Biotechnology
Nanomedicine

Background:

Accurate detection of pathogens like E. coli O157:H7 is crucial for public health.
Existing methods often require lengthy procedures, signal amplification, or sample enrichment.

Purpose of the Study:

To develop a rapid, sensitive, and specific bioassay for detecting single bacterial cells and DNA.
To showcase the potential of dye-doped silica nanoparticles in biodetection systems.

Main Methods:

Utilized antibody-conjugated dye-doped silica nanoparticles (NPs) for specific bacterial capture.
Employed antibody-antigen recognition for quantitative detection of E. coli O157:H7.
Demonstrated DNA detection using dye-doped silica NPs at sub-fentomolar concentrations.

Main Results:

Achieved accurate determination of single bacterial cells within 20 minutes.
Demonstrated specific and quantitative detection of E. coli O157:H7 from beef samples.
Successfully detected DNA at sub-fentomolar concentrations without signal amplification.

Conclusions:

Dye-doped silica nanoparticles offer a versatile platform for ultrasensitive biodetection.
This bionanotechnology approach has significant potential for medical diagnostics and infectious agent detection.