Curve tracking for rapid imaging in AFM
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Updated: Jul 8, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
Published on: August 11, 2020
1Department of Aerospace and Mechanical Engineering, Boston University, Boston, MA 02215, USA. sanderss@bu.edu
Abstract:
A high-level feedback control approach for rapid imaging in atomic force microscopy is presented. The algorithms are designed for samples which are string-like, such as biopolymers, and for boundaries. Rather than the simple raster-scan pattern, data from the microscope are used in real-time to steer the tip along the sample, drastically reducing the area to be imaged. An order-of-magnitude reduction in the time to acquire an image is possible. The technique is illustrated through simulations and through physical experiments.

