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Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Related Experiment Video

Updated: Jul 8, 2026

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
09:45

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Published on: August 10, 2017

Serial analysis of gene expression (SAGE).

Fred van Ruissen1, Frank Baas

  • 1Neurogenetics Laboratory, Academic Medical Center, Amsterdam, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|January 26, 2008
PubMed
Summary

Serial Analysis of Gene Expression (SAGE) is a powerful tool for studying gene expression profiles and discovering new transcripts across various model systems. This method requires no prior genomic information, facilitating broad research applications.

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Serial Analysis of Gene Expression (SAGE) was introduced in 1995.
  • SAGE is a versatile technique for gene expression analysis.
  • It does not require prior knowledge of the target genome.

Purpose of the Study:

  • To describe the SAGE procedure.
  • To highlight its utility in generating gene expression profiles.
  • To demonstrate its capability in identifying novel transcripts.

Main Methods:

  • The chapter details the SAGE methodology.
  • Focuses on its application in gene expression profiling.
  • Explains its use for transcript discovery.

Main Results:

More Related Videos

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress
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Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress

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A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
07:09

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

Related Experiment Videos

Last Updated: Jul 8, 2026

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
09:45

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Published on: August 10, 2017

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress
05:22

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress

Published on: July 29, 2022

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
07:09

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

  • SAGE enables the creation of comprehensive gene expression profiles.
  • The technique facilitates the identification of previously unknown transcripts.
  • SAGE data is readily shareable and accessible via online databases.

Conclusions:

  • SAGE is an adaptable tool for gene expression studies in diverse systems.
  • It serves dual purposes: profiling and novel transcript identification.
  • The accessibility of SAGE data promotes collaborative research.