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Affinity chromatography of Escherichia coli (m5U54)-methyltransferase on tRNA-agarose
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Protein Expression and Purification
|February 1, 1991
Abstract:
tRNA-agarose was prepared by condensing periodate-oxidized tRNA to an agarose matrix containing hydrazide functional groups. The tRNA-agarose was used to take partially purified tRNA (m5U54)-methyltransferase to homogeneity. The method is simple and reproducible and gives high yields.