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Updated: Jul 8, 2026
Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
Published on: July 30, 2011
Isolation and analysis of genes specifically expressed during basidiomatal development in Antrodia cinnamomea by
Fang-Hua Chu1, Yi-Ru Lee, Shu-Jen Chou
1School of Forestry and Resource Conservation, National Taiwan University, Taipei, Taiwan.
Abstract:
cDNAs specifically expressed at the basidiome stage were isolated by using PCR-selected cDNA subtraction in order to study gene regulation during porous-hymenium basidiomatal formation in Antrodia cinnamomea. blastx results suggested that most of the expressed sequence tags (52.4-69.5%) had no significant protein homology to genes from other published living things. cDNAs particularly expressed at different growing conditions were identified using cDNA microarray analysis. Reverse transcriptase PCR analyses confirmed that the clone putative to P-type ATPase, various cytochrome P450s and some unknown genes were abundant at natural basidiomes while endoglucanase was abundant at the tissue from artificial medium.
