Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Tyrosine kinase mutation and acute myeloid leukemia with T (8; 21)].

Zhongguo shi yan xue ye xue za zhi·2009

Same author

[Clinical study on 10 cases of pancytopenia selectively treated by cyclosporine a].

Zhongguo shi yan xue ye xue za zhi·2009

Same author

Melatonin protects against oxidative damage in a neonatal rat model of bronchopulmonary dysplasia.

World journal of pediatrics : WJP·2009

Same author

Ion trap mass analysis at high pressure: a theoretical view.

Journal of the American Society for Mass Spectrometry·2009

Same author

Coordinating to three histidine residues: Cu(II) promotes oligomeric and fibrillar amyloid-beta peptide to precipitate in a non-beta aggregation manner.

Journal of Alzheimer's disease : JAD·2009

Same author

[Effect of melatonin on hyperoxia-induced oxidant/antioxidant imbalance in the lung of neonatal rats with chronic lung disease].

Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics·2009

Search research articles

Related Experiment Video

Updated: Jul 8, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

EzArray: a web-based highly automated Affymetrix expression array data management and analysis system.

Yuerong Zhu¹, Yuelin Zhu, Wei Xu

¹Research and Development, BioInfoRx, Inc,, Middleton, WI 53562, USA. ron@bioinforx.com

BMC Bioinformatics

|January 26, 2008

Summary

This summary is machine-generated.

EzArray is a web-based system simplifying Affymetrix microarray data management and analysis for researchers. It offers automated tools for efficient data organization, preprocessing, and differential gene expression analysis, even for novice users.

More Related Videos

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Related Experiment Videos

Last Updated: Jul 8, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Area of Science:

Bioinformatics
Computational Biology
Genomics

Background:

Microarray experiments are widely used in life sciences but present significant data management and analysis challenges for biologists.
Existing microarray analysis tools often demand advanced mathematical, statistical, and computational expertise.
There is a high demand for user-friendly programs to re-analyze the growing volume of microarray data available in public databases.

Purpose of the Study:

To develop a web-based system for managing and analyzing Affymetrix expression array data.
To provide an accessible platform for researchers, including novices, to perform initial microarray data analysis.
To enable efficient re-analysis of published microarray data from public repositories.

Main Methods:

EzArray is a web-based system that organizes microarray data into projects.
It offers online analysis with predefined or custom procedures, including data preprocessing and differential gene expression detection.
The system integrates with the Gene Expression Omnibus (GEO) for instantaneous re-analysis of published data.

Main Results:

EzArray provides an automated and highly optimized analysis pipeline, enabling quick initial analysis for users with limited prior knowledge.
The system ensures maximum reproducibility by allowing users to download input files, analysis parameters, and executed scripts.
EzArray facilitates efficient organization and instant analysis of microarray data, supporting both new and previously published datasets.

Conclusions:

EzArray is a novel system for Affymetrix expression array data analysis and sharing, offering user-friendly tools for re-analysis.
It empowers both novice and experienced users to perform initial microarray data analysis efficiently.
EzArray is a valuable resource for facilities offering microarray services and for collaborative research laboratories, and is freely available online.