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Related Concept Videos

In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jul 8, 2026

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
08:36

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

TILLING to detect induced mutations in soybean.

Jennifer L Cooper1, Bradley J Till, Robert G Laport

  • 1Fred Hutchinson Cancer Research Center, Seattle, WA 98107, USA. jlcooper@fhcrc.org

BMC Plant Biology
|January 26, 2008
PubMed
Summary

This study developed four mutagenized soybean populations for gene function studies using Targeting Induced Local Lesions IN Genomes (TILLING). Researchers identified 116 mutations, demonstrating soybean

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Area of Science:

  • Plant genetics
  • Molecular biology
  • Agricultural science

Background:

  • Soybean (Glycine max L. Merr.) is a vital global source of protein and oil.
  • Limited tools currently exist for investigating soybean gene function.
  • Chemical mutagenesis offers a method for generating mutations for screening.

Purpose of the Study:

  • To apply the Targeting Induced Local Lesions IN Genomes (TILLING) strategy to soybean.
  • To develop and characterize mutagenized soybean populations for gene discovery.
  • To optimize mutation screening in soybean's complex genome.

Main Methods:

  • Applied TILLING to four mutagenized soybean populations (3 EMS, 1 NMU).
  • Screened seven genetic targets per population.
  • Developed a method using restriction enzymes to eliminate extraneous PCR targets.

Main Results:

  • Discovered a total of 116 induced mutations across the populations.
  • Observed varying mutation densities, with some populations reaching approximately 1/140 kb.
  • Identified four additional mutant alleles by pretreating DNA with a restriction enzyme.

Conclusions:

  • Soybean is amenable to high-throughput mutation discovery using TILLING.
  • Four independent populations with significant mutation density were successfully developed.
  • An improved screening method enhances mutation identification in polyploid genomes.