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Updated: Jul 8, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
Published on: March 8, 2016
Combination of two hydrophilic interaction chromatography methods that facilitates identification of
Göran Karlsson1, Elin Swerup, Helena Sandberg
1Octapharma AB, SE-11275 Stockholm, Sweden. goran.karlsson@octapharma.se
Abstract:
Hydrophilic interaction chromatography (HILIC) is used to separate 2-aminobenzamide- (2-AB) labeled N-linked oligosaccharides. The glycans of the model protein, bovine fetuin, are identified following comparison of elution patterns of seven 2-AB-labeled glycan standards, of which two are of the high-mannose type and five are of the complex type. The combination of two HILIC methods, using an Amide-80 column, having different resolutions and selectivities, markedly facilitates the identification of the fetuin glycans. These HILIC methods are suitable for obtaining glycan profiles of complex mixtures.
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