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Isolation and characterization of glycocalyx in Streptomyces aureofaciens
J Shabalin1, I Benes, Z Hostálek
1Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Moscow Region, USSR.
Folia Microbiologica
|January 1, 1991
Abstract:
The surface layer, considered to be glycocalyx according to electron-microscopic observations, was separated from a low-production strain of Streptomyces aureofaciens by solubilization with urea and subsequent sonication. The isolation procedure was developed using various agents; neutral phosphatase served as a marker indicating the amount of the material released. The peripheral structure consisted predominantly of glycoprotein and differed from S-layers.