Modulation of CaV2.3 calcium channel currents by eugenol
1Department of Physiology, School of Dentistry, Seoul National University, 28-2 Yeongeon-Dong, Chongno-Ku, Seoul, 110-749, Korea.
Journal of Dental Research
|January 26, 2008
Summary
Eugenol, a dental analgesic, inhibits Ca(V)2.3 calcium channels, distinct from capsaicin
Area of Science:
- Pharmacology
- Neuroscience
- Molecular Biology
Background:
- Eugenol is a common dental analgesic.
- Its analgesic effects are partly due to inhibition of Ca(V)2.2 and sodium channels.
- The role of Ca(V)2.3 channels in eugenol's action is unknown.
Purpose of the Study:
- To investigate if eugenol modulates Ca(V)2.3 calcium channels.
- To determine the mechanism of eugenol's action on Ca(V)2.3 channels.
- To compare eugenol's mechanism with that of capsaicin.
Main Methods:
- Whole-cell patch-clamp technique.
- Heterologous expression system using E52 cell line stably expressing human Ca(V)2.3 channels.
- Comparison between cells with and without TRPV1 expression.
Main Results:
- Eugenol inhibited Ca(V)2.3 calcium currents.
- TRPV1 expression did not affect the extent of inhibition by eugenol.
- Eugenol's inhibition of Ca(V)2.3 channels is independent of TRPV1, unlike capsaicin.
Conclusions:
- Eugenol modulates Ca(V)2.3 calcium channels through a mechanism distinct from capsaicin.
- Eugenol's inhibition of Ca(V)2.3 channels may contribute to its analgesic properties.
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