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Updated: Jul 8, 2026
10:31
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
Novel biphasic role for lymphocytes revealed during resolving inflammation
Ravindra Rajakariar1, Toby Lawrence, Jonas Bystrom
1Department of Experimental Medicine and Translational Therapeutics, William Harvey Research Institute, London, United Kingdom.
Blood
|January 26, 2008
Summary
T and B lymphocytes regulate acute inflammation and its resolution by controlling cytokine synthesis. While disappearing during inflammation, repopulating lymphocytes are crucial for modulating secondary infections and immune responses.
Area of Science:
- Immunology
- Cell Biology
Background:
- The classic view of acute inflammation focuses on polymorphonuclear leukocytes (PMNs) and macrophages.
- The role of lymphocytes in acute inflammation and resolution is often overlooked, despite their innate immune-regulatory properties.
Purpose of the Study:
- To investigate the role of T and B lymphocytes in acute peritoneal inflammation and its resolution.
- To understand how lymphocytes modulate immune responses during inflammation and upon secondary challenge.
Main Methods:
- Utilized lymphocyte-deficient RAG1(-/-) mice to assess lymphocyte function.
- Administered prostaglandin D(2) to induce lymphocyte disappearance during inflammation.
- Studied chronic granulomatous disease models (gp91phox(-/-) mice) to evaluate responses to superinfection.
Main Results:
- Peritoneal T/B lymphocytes control PMN trafficking by regulating cytokine synthesis.
- Lymphocytes disappear during acute inflammation but repopulate during resolution.
- Repopulating lymphocytes are essential for modulating responses to secondary infections, as seen in gp91phox(-/-) mice.
Conclusions:
- Lymphocytes transition from regulating acute inflammation to orchestrating responses during resolution.
- Lymphocyte repopulation is critical for preventing exaggerated immune responses to secondary infections.
- Findings in mice are relevant to human peritonitis, highlighting a dynamic role for lymphocytes in immune responses.
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