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Soil Sampling and Isolation of Entomopathogenic Nematodes (Steinernematidae, Heterorhabditidae)
Published on: July 11, 2014
Nocardioides hankookensis sp. nov., isolated from soil
Jung-Hoon Yoon1, So-Jung Kang, Mi-Hwa Lee
1Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea. jhyoon@kribb.kr
International Journal of Systematic and Evolutionary Microbiology
|January 26, 2008
Summary
A novel bacterial species, Nocardioides hankookensis, was identified from Korean soil. This Gram-positive rod is phylogenetically related to other Nocardioides species but distinct enough to be classified as new.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- The genus Nocardioides comprises Gram-positive bacteria with diverse ecological roles.
- Accurate bacterial classification is crucial for understanding microbial diversity and function.
Purpose of the Study:
- To taxonomically characterize a novel bacterial isolate, strain DS-30(T), from Korean soil.
- To determine if strain DS-30(T) represents a new species within the Nocardioides genus.
Main Methods:
- Polyphasic taxonomic study including 16S rRNA gene sequencing.
- Phylogenetic analysis and DNA-DNA hybridization.
- Chemotaxonomic analysis (cell wall, menaquinone, fatty acids) and phenotypic characterization.
Main Results:
- Strain DS-30(T) is a Gram-positive, non-motile rod, growing optimally at pH 6.0-7.0 and 25°C.
- Phylogenetic analysis showed 98.1-98.3% 16S rRNA gene similarity to Nocardioides aquiterrae and Nocardioides pyridinolyticus, but <96.8% to other Nocardioides species.
- DNA-DNA relatedness was low (19-22%) with related species, and phenotypic differences were observed.
Conclusions:
- Strain DS-30(T) represents a novel species of the genus Nocardioides.
- The proposed name for this new species is Nocardioides hankookensis sp. nov.
- The type strain is DS-30(T) (=KCTC 19246(T) =CCUG 54522(T)).
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