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Updated: Jul 8, 2026
An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
A concern regarding the current confusion with the human homolog of mouse Np95, ICBP90/UHRF1
Motoko Unoki1, Christian Bronner, Marc Mousli
1Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892-4258, USA. unokim@mail.nih.gov
Abstract:
ICBP90/UHRF1, which is overexpressed in cancer cells and is down-regulated by p53, possesses a methylated CpG binding affinity and binds to the methylated promoters of tumor suppressor genes in cancer cells with HDAC1 and DNMT1, suggesting suppression of these genes and maintenance of methylation status which leads to carcinogenesis. Recently, it was reported that the human homolog of Np95 is different from ICBP90 but not from UHRF1. Because UHRF1 is the gene symbol of ICBP90, the claim is a little confusing; that is, UHRF1 and ICBP90 are identical. Because the previously published genomic structure of the ICBP90 gene needed to be revised and the registered ICBP90 sequence (AF129507) contains two rare polymorphisms or sequence errors, we think that confusion could occur. Here we show the revised ICBP90 gene structure and 366 polymorphisms in this gene. Our conclusion is that the human homolog of Np95 is ICBP90, whose gene symbol is UHRF1.
Insights
The study clarifies that ICBP90 and UHRF1 are the same gene, identified as the human homolog of Np95. Researchers revised the ICBP90 gene structure and identified numerous polymorphisms, resolving previous confusion.
Area of Science:
- Molecular Biology
- Cancer Research
- Genetics
Background:
- The protein ICBP90/UHRF1 is overexpressed in cancer, binds methylated DNA, and represses tumor suppressor genes, contributing to carcinogenesis.
- Previous reports caused confusion regarding the identity of the human homolog of Np95, specifically its relationship with ICBP90 and UHRF1.
Purpose of the Study:
- To resolve the confusion surrounding the identity of the human homolog of Np95.
- To revise the genomic structure of the ICBP90 gene and identify its polymorphisms.
- To confirm the identity of ICBP90 as the human homolog of Np95, with UHRF1 as its gene symbol.
Main Methods:
- Revision of the previously published genomic structure of the ICBP90 gene.
- Identification and characterization of 366 polymorphisms within the ICBP90 gene.
- Sequence analysis to compare ICBP90 with UHRF1 and the human homolog of Np95.
Main Results:
- The gene symbol UHRF1 is identical to ICBP90.
- The revised genomic structure of ICBP90 was established.
- A total of 366 polymorphisms were identified in the ICBP90 gene.
- The human homolog of Np95 was confirmed to be ICBP90/UHRF1.
Conclusions:
- ICBP90 and UHRF1 are the same gene.
- The human homolog of Np95 is ICBP90, with the gene symbol UHRF1.
- The revised gene structure and identified polymorphisms clarify previous ambiguities.
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