Related Experiment Video
Updated: Jul 8, 2026
09:18
Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo
Published on: April 21, 2022
Lessons from the stem cell proteome
Bernd Gesslbauer1, Evelyn Krenn, Christoph Zenzmaier
1Institute of Pharmaceutical Sciences, University of Graz, A-8010 Graz, Austria.
Current Stem Cell Research & Therapy
|January 29, 2008
Summary
Investigating the stem cell proteome using advanced techniques reveals dynamic protein expression. Stem cell protein patterns reflect intrinsic metabolism and environmental influences, impacting diagnostic and therapeutic applications.
Area of Science:
- Proteomics
- Stem Cell Biology
- Molecular Diagnostics
Background:
- The cellular proteome serves as a molecular fingerprint of gene expression.
- Monitoring stem cell expansion and differentiation via proteomics offers diagnostic and therapeutic potential.
Purpose of the Study:
- To comprehensively analyze the proteome of umbilical cord blood-derived CD34+/AC133+ stem cells.
- To understand the dynamic nature of the stem cell proteome.
Main Methods:
- Two-dimensional (2-D) gel electrophoresis and 2-D chromatography for protein separation.
- Nano-high-performance liquid chromatography (HPLC) coupled to an ion trap mass spectrometer for protein identification after tryptic digestion.
- Extensive bioinformatic analysis of identified proteins.
Main Results:
- Revealed a dynamic stem cell proteome with heterogeneous protein expression patterns.
- Identified a core set of stem cell-specific housekeeping proteins.
- Observed a significant number of proteins influenced by environmental stimuli and sample history.
Conclusions:
- Stem cell proteome analysis is complex due to dynamic expression influenced by intrinsic and extrinsic factors.
- Snapshot proteomic views reflect both cellular metabolism and sample history.
- Understanding proteomic heterogeneity is crucial for stem cell diagnostics and therapeutics.
More Related Videos
Related Concept Videos
01:26Stem Cell Niche
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
01:17Stem Cell Culture
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
00:58Embryonic Stem Cells
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
01:21Stem Cell Therapy for Tissue Regeneration
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...