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Updated: Jul 8, 2026

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
Published on: February 10, 2023
The hypervariable D3 domain of Salmonella flagellin is an autonomous folding unit
Anett Sebestyén1, Adél Muskotál, Barbara M Végh
1Research Institute for Technical Physics and Materials Science, Hungarian Academy of Sciences, Konkoly Thege u. 29-33, H-1121 Budapest, Hungary.
Abstract:
The hypervariable D3 domain of Salmonella flagellin, composed of the 190-285 segment, is the major determinant of flagellar antigenicity. D3 was cloned and overexpressed in E. coli. Although previous studies concluded that D3 is stabilized by interactions with the D2 domain, our calorimetric experiments have revealed that isolated D3 has a stable tertiary structure which is highly resistant against proteolytic digestion. Repeated heating experiments demonstrated that unfolding of D3 is reversible. Its small size and stable structure makes D3 a promising protein scaffold for the development of artificial binding proteins by directed evolution.
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