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Reproductive Techniques for Ovarian Monitoring and Control in Amphibians
04:37

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Published on: May 12, 2019

Modulators of Bufo arenarum ovulation.

Inés Ramos1, Susana B Cisint, Claudia A Crespo

  • 1Instituto Superior de Investigaciones Biológicas, Departamento de Biología del Desarrollo, Universidad Nacional de Tucumán, Argentina, Chacabuco 461, Tucumán, Argentina. inramos@fbqf.unt.edu.ar

Zygote (Cambridge, England)
|January 29, 2008
PubMed
Summary

This study shows that homologous pituitary homogenate (HPH) effectively induces ovulation in Bufo arenarum. Prostaglandin F2alpha (PGF2alpha) enhances ovulation, while Prostaglandin E1 (PGE1) inhibits it.

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Area of Science:

  • Reproductive Biology
  • Amphibian Endocrinology
  • Ovarian Physiology

Background:

  • Ovulation is a critical reproductive event regulated by hormonal signals.
  • The roles of pituitary hormones and prostaglandins in amphibian ovulation require further elucidation.
  • Bufo arenarum serves as a model organism for studying vertebrate reproductive processes.

Purpose of the Study:

  • To investigate the in vitro ovulatory response of Bufo arenarum ovaries to various hormonal stimuli.
  • To determine the involvement of prostaglandins (PGF2alpha, PGE1) and progesterone in pituitary-induced ovulation.
  • To examine the synergistic or inhibitory effects of these compounds on ovulation.

Main Methods:

  • In vitro incubation of Bufo arenarum ovary samples obtained during the breeding season.
  • Stimulation with homologous pituitary homogenate (HPH), progesterone, PGF2alpha, and PGE1.
  • Dose-response experiments and assessment of ovulated oocytes.
  • Use of cyclooxygenase inhibitors (indomethacin, diclofenac sodium) to probe prostaglandin involvement.

Main Results:

  • Ovulatory response was dose-dependent for all tested inducers.
  • HPH treatment yielded the highest percentage of ovulated oocytes.
  • PGF2alpha demonstrated a synergistic potentiating effect when combined with suboptimal doses of HPH or progesterone.
  • Cyclooxygenase inhibitors significantly reduced HPH-induced ovulation, confirming prostaglandin involvement.
  • PGE1 exhibited an inhibitory effect on HPH-induced ovulation.

Conclusions:

  • Pituitary action in Bufo arenarum ovulation involves prostaglandins.
  • PGF2alpha plays a crucial role in potentiating ovulation, likely through synergistic mechanisms.
  • PGE1 appears to inhibit the ovulatory process, contrary to its expected role.
  • These findings contribute to understanding the complex hormonal regulation of ovulation in amphibians.