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Updated: Jul 7, 2026
Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
Potato glycoalkaloids in soil-optimising liquid chromatography-time-of-flight mass spectrometry for quantitative
Pia H Jensen1, René K Juhler, Nikoline J Nielsen
1Department of Natural Sciences, Faculty of Life Sciences (LIFE), University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark. phj@life.ku.dk
Abstract:
Potato glycoalkaloids are produced in high amounts in potato fields during the growth season and losses to soil potentially impact shallow groundwater and via tiles to fresh water ecosystems. A quantitative liquid chromatography-electrospray ionization time-of-flight mass spectrometry (LC-ESI-TOF-MS) method for determination and quantification of potato glycoalkaloids and their metabolites in aqueous soil extracts was developed. The LC-ESI-TOF-MS method had linearities up to 2000microg/L for alpha-solanine and alpha-chaconine and up to 760microg/L for solanidine. No matrix effect was observed, and the detection limits found were in the range 2.2-4.7microg/L. The method enabled quantification of the potato glycoalkaloids in environmental samples.
