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Updated: Jul 7, 2026
Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle
Published on: August 5, 2012
Curcumin attenuates ischemia-reperfusion injury in rat testis
Si-Ming Wei1, Zhi-Zhong Yan, Jian Zhou
1Department of Urology, Third Affiliated Hospital of Hangzhou City, Zhejiang Chinese Medical University, Hangzhou City, Zhejiang Province, China. wsm1971@hotmail.com
Objective:
To evaluate the effect of curcumin, a potent antioxidant, on testicular ischemia-reperfusion injury caused by overgeneration of reactive oxygen species (ROS) after testicular torsion-detorsion.
Design:
Controlled experimental study using rats.
Setting:
Research laboratory.
Animal(S):
Sixty adult male Sprague-Dawley rats.
Intervention(S):
Rats in the control group underwent a sham operation of the left testis. In the torsion-detorsion group, the left testis was rotated 720 degrees for 2 hours. Rats in treatment group received the same surgical procedure as the torsion-detorsion group, but curcumin was administered IV at repair of testicular torsion.
Main Outcome Measure(S):
Testicular activity of xanthine oxidase, which catalyzes production of ROS; malondialdehyde level (an indicator of ROS content); protein expression level of heme oxygenase-1, which catalyzes antioxidant generation; and spermatogenesis.
Result(S):
Unilateral testicular torsion-detorsion caused significant increases in xanthine oxidase activity, malondialdehyde level, and heme oxygenase-1 protein expression level and caused a significant decrease in testicular spermatogenesis in ipsilateral testes. The rats treated with curcumin had significant decreases in xanthine oxidase activity and malondialdehyde level and had significant increases in heme oxygenase-1 protein expression level and testicular spermatogenesis in ipsilateral testes, compared with the torsion-detorsion group.
Conclusion(S):
The curcumin exerts a protective effect on testicular ischemia-reperfusion injury.
