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Updated: Jul 7, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

The current status of cDNA cloning.

Matthias Harbers¹

¹DNAFORM, Inc., Leading Venture Plaza 2, 75-1 Ono-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0046, Japan. matthias.harbers@dnaform.jp

|January 29, 2008

Summary

Complementary DNA (cDNA) cloning technologies have advanced significantly, enabling large-scale genetic information studies. New methods are crucial for discovering rare transcripts and novel RNA families in complex genomes.

Area of Science:

Molecular Biology
Genomics
RNA Biology

Background:

Complementary DNA (cDNA) cloning is a foundational molecular biology technique.
Advances over 30 years have enabled large cDNA collections, crucial for understanding genetic information utilization.
Discovery of noncoding RNAs spurred new approaches for short RNA cloning.

Purpose of the Study:

To review the current status of cDNA cloning technologies.
To explore new challenges and possibilities in cDNA cloning.
To facilitate the discovery and characterization of novel RNA families.

Main Methods:

Review of established and emerging cDNA cloning methodologies.
Analysis of technological advancements in RNA and cDNA library construction.

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Use of Alu Element Containing Minigenes to Analyze Circular RNAs

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A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

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Discussion of strategies for identifying rare transcripts.

Main Results:

cDNA cloning has evolved to support comprehensive genetic studies.
New challenges arise from the vastness of the transcribed genome.
Emerging techniques are essential for uncovering rare and novel RNA species.

Conclusions:

Current cDNA cloning methods face challenges in capturing the full transcriptome.
Further innovation is needed to discover and functionally characterize rare transcripts and new RNA families.
This review highlights the dynamic nature of cDNA cloning and its future directions.