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Genetic exchange between kingdoms.

G F Sprague1

  • 1Institute of Molecular Biology, University of Oregon, Eugene 97403.

Current Opinion in Genetics & Development
|December 11, 1991
PubMed
Summary

Trans-kingdom bacterial conjugation between bacteria and yeasts occurs in labs. Recent evidence suggests these events, including horizontal gene transfer, may happen in nature, challenging our understanding of microbial evolution.

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Area of Science:

  • Microbiology
  • Evolutionary Biology
  • Genetics

Background:

  • Bacterial conjugation is a primary mechanism for horizontal gene transfer (HGT) in prokaryotes.
  • Trans-kingdom conjugation, particularly between bacteria and eukaryotes like yeast, has been demonstrated experimentally.
  • The natural occurrence of such inter-kingdom gene transfer events remains largely unconfirmed, with few exceptions like Agrobacterium-plant interactions.

Purpose of the Study:

  • To investigate the potential for natural occurrence of bacterial conjugation events involving yeasts.
  • To explore the broader implications of inter-kingdom horizontal gene transfer in microbial evolution.
  • To assess whether observed laboratory phenomena reflect natural biological processes.

Main Methods:

  • Laboratory experiments demonstrating bacterial conjugation with evolutionarily divergent yeast species.
  • Analysis of gene and protein sequences to identify potential instances of past horizontal gene transfer between kingdoms.
  • Comparative genomics and phylogenetic analysis to infer gene flow across kingdoms.

Main Results:

  • Successful bacterial conjugation with two distinct yeast species was achieved under laboratory conditions.
  • Sequence analysis revealed several putative instances of gene transfer between bacteria and eukaryotes.
  • These findings suggest that horizontal gene transfer across phylogenetic kingdoms might be more prevalent than previously thought.

Conclusions:

  • Bacterial conjugation with yeasts is feasible and can be studied experimentally.
  • Emerging evidence from sequence data supports the occurrence of natural trans-kingdom horizontal gene transfer.
  • This phenomenon could significantly impact the evolutionary trajectories of both prokaryotic and eukaryotic organisms.

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