Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Single-centre experience with peptide receptor radionuclide therapy for neuroendocrine tumours (NETs): results using a theranostic molecular imaging-guided approach.

Journal of cancer research and clinical oncology·2023

Same author

Partial volume effect in SPECT & PET imaging and impact on radionuclide dosimetry estimates.

Asia Oceania journal of nuclear medicine & biology·2023

Same author

Practical learning through radiation physics problem solving.

Radiography (London, England : 1995)·2022

Same author

Theranostic SPECT reconstruction for improved resolution: application to radionuclide therapy dosimetry.

EJNMMI physics·2021

Same author

Obesity alters the topographical distribution of ventilation and the regional response to bronchoconstriction.

Journal of applied physiology (Bethesda, Md. : 1985)·2019

Same author

Diagnosis and Management of Mild Traumatic Brain Injury in Children: A Systematic Review.

JAMA pediatrics·2018

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Robotized Testing of Camera Positions to Determine Ideal Configuration for Stereo 3D Visualization of Open-Heart Surgery

Robotized Testing of Camera Positions to Determine Ideal Configuration for Stereo 3D Visualization of Open-Heart Surgery

Published on: August 12, 2021

Fully three-dimensional reconstruction for a PET camera with retractable septa.

D W Townsend¹, A Geissbuhler, M Defrise

¹Div. of Nucl. Med., Univ. Hospital, Geneva.

IEEE Transactions on Medical Imaging

|January 1, 1991

Summary

A new 3-D reconstruction algorithm enhances Positron Emission Tomography (PET) imaging. Retractable septa on the Siemens/CTI 953B camera significantly boost data acquisition rates for improved 3-D PET scans.

More Related Videos

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Related Experiment Videos

Last Updated: Jul 7, 2026

Robotized Testing of Camera Positions to Determine Ideal Configuration for Stereo 3D Visualization of Open-Heart Surgery

Robotized Testing of Camera Positions to Determine Ideal Configuration for Stereo 3D Visualization of Open-Heart Surgery

Published on: August 12, 2021

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Area of Science:

Medical Imaging
Nuclear Medicine
Computational Science

Background:

Positron Emission Tomography (PET) imaging relies on accurate data reconstruction.
Existing PET systems may have limitations in data acquisition rates and reconstruction fidelity.

Purpose of the Study:

To develop and validate a fully 3-D reconstruction algorithm for a specific PET camera.
To assess the impact of retractable septa on system count rates and data quality.

Main Methods:

Developed a 3-D filtered backprojection algorithm tailored for multi-angle sinogram data.
Utilized forward projection to obtain missing sinogram data due to tomograph geometry.
Employed a voxel-driven procedure for backprojection into a 128x128x31 voxel volume.

Main Results:

Septa retraction increased system count rates by factors of 7.8 (including scatter) and 4.7 (scatter subtracted).
The 3-D reconstruction algorithm was validated using simulation, phantom, and clinical data.
The algorithm effectively reconstructs data from the 16-ring PET camera.

Conclusions:

The developed 3-D reconstruction algorithm is effective for the Siemens/CTI 953B PET camera.
Retractable septa technology significantly improves PET system performance.
This advancement holds potential for enhanced diagnostic accuracy in PET imaging.