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Updated: Jul 7, 2026

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
11:22

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Published on: January 30, 2018

An error analysis for two-state protein-folding kinetic parameters and phi-values: progress toward precision by

Eva S Cobos1, Adela M Candel, Jose C Martinez

  • 1Department of Physical Chemistry and Institute of Biotechnology, Faculty of Sciences, University of Granada, 18071 Granada, Spain.

Biophysical Journal
|January 29, 2008
PubMed
Summary

This study refines phi-value analysis for protein folding transition states using alphaspectrin SH3 domain kinetic data. It establishes error propagation methods, yielding a precise 25% accuracy for phi-values.

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