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Related Experiment Videos

Human embryonic stem cell derivation from poor-quality embryos.

Paul H Lerou1, Akiko Yabuuchi, Hongguang Huo

  • 1Division of Newborn Medicine, Brigham & Women's Hospital, Boston, Massachusetts 02115, USA.

Nature Biotechnology
|January 29, 2008
PubMed
Summary
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Clinically useless embryos from in vitro fertilization can yield human embryonic stem (hES) cells. Blastocyst-stage embryos are a reliable source for normal hES cell lines, unlike arrested or fragmented ones.

Area of Science:

  • Embryology
  • Stem Cell Biology
  • Reproductive Medicine

Background:

  • Embryos with poor morphology are often discarded during in vitro fertilization (IVF).
  • The potential of these discarded embryos as a source for human embryonic stem (hES) cells is largely unexplored.
  • Identifying reliable sources of hES cells is crucial for regenerative medicine and disease research.

Purpose of the Study:

  • To investigate the correlation between the developmental stage of poor-quality human embryos and the success rate of deriving human embryonic stem (hES) cell lines.
  • To determine if specific embryonic stages are more amenable to hES cell derivation, even from morphologically compromised embryos.

Main Methods:

  • Analysis of discarded embryos from IVF procedures based on morphological criteria.

Related Experiment Videos

  • Correlation of embryo developmental stage (e.g., fragmentation, arrest, blastocyst) with hES cell line establishment.
  • Characterization of derived hES cell lines for pluripotency and normality.

    • Main Results:

    • A statistical correlation was observed between embryo developmental stage and hES cell line yield.
    • Early-arrested or highly fragmented embryos rarely yielded viable hES cell lines.
    • Embryos reaching the blastocyst stage, despite poor morphology, proved to be a robust source of normal hES cells.

    Conclusions:

    • The developmental stage of an embryo is a critical factor in successful human embryonic stem (hES) cell line derivation, even when morphology is poor.
    • Blastocyst-stage embryos represent a valuable, often overlooked, resource for generating normal hES cells.
    • This finding could improve the efficiency of hES cell banking and research by utilizing previously discarded embryos.