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Updated: Jul 7, 2026

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
Intranuclear nucleolin distribution during cell cycle progression in human invasive ductal breast carcinomas in
Marek Masiuk1, Elzbieta Urasinska, Wenancjusz Domagala
1Department of Pathology, Pomeranian Medical University, 71-252 Szczecin, Poland. mmasiuk@med.pam.szczecin.pl
Background:
Nucleolin (NU) can be found in the nucleus in aggregates and in a diffused form. The expression and distribution of these two intranuclear pools of nucleolin was compared in estrogen receptor (ER)-positive and -negative invasive ductal breast carcinomas in relation to cell cycle phases.
Materials And Methods:
Using a laser scanning cytometer NU was measured within the whole nucleus, within nucleolin aggregates (NUA) and within the NUA-free karyoplasm. The G1-, S- and G2M-phase cell subpopulations were distinguished on DNA histograms.
Results:
The nuclei of ER-positive and ER-negative breast carcinomas in the G2M-phase differed in the expression of NU in the NUA and in the NUA-free karyoplasm, whereas in the S-phase they only differed in the NUA-free karyoplasm expression. In ER-positive carcinomas NU levels rose from G1 to G2M in both the NUA and the NUA-free karyoplasm, whereas in ER-negative carcinomas the NU levels increased only between G1/S in the NUA and between S/G2M in the NUA-free karyoplasm.
Conclusion:
There are significant differences in nucleolin expression and localization during cell cycle progression depending on ER status.
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