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Related Experiment Video

Updated: Jul 7, 2026

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

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Published on: November 8, 2024

Circulating angiogenic factors in singleton vs multiple-gestation pregnancies.

Sharon E Maynard¹, Tiffany A Moore Simas, Matthew J Solitro

¹Department of Medicine, George Washington University Medical Center, Washington, DC, USA. smaynard@mfa.gwu.edu

American Journal of Obstetrics and Gynecology

|January 30, 2008

Summary

Women with multiple gestation pregnancies have altered levels of angiogenic factors, specifically higher placental soluble fms-like tyrosine kinase-1, which may explain their increased preeclampsia risk.

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Guide Wire Assisted Catheterization and Colored Dye Injection for Vascular Mapping of Monochorionic Twin Placentas

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Published on: September 5, 2011

Area of Science:

Reproductive biology
Maternal-fetal medicine
Endocrinology

Background:

Preeclampsia is a serious pregnancy complication.
Multiple gestation pregnancies are at higher risk for preeclampsia.
Soluble fms-like tyrosine kinase-1 (sFLT1) is implicated in preeclampsia pathogenesis.

Purpose of the Study:

To investigate serum angiogenic factor levels in women with multiple gestation pregnancies.
To compare angiogenic factor profiles between multiple and high-risk singleton pregnancies.
To assess the role of angiogenic factors in preeclampsia risk associated with multiple gestations.

Main Methods:

Serial serum samples collected from 101 high-risk pregnant women (22-36 weeks' gestation).
Quantification of soluble fms-like tyrosine kinase-1 (sFLT1) and placental growth factor (PlGF) using ELISA.
Exclusion of women who developed preeclampsia or gestational hypertension.

Main Results:

Maternal sFLT1 levels were elevated in multiple gestation (n=20) versus high-risk singleton (n=81) pregnancies across all gestational age ranges.
Maternal PlGF was significantly higher in multiple gestations before 31 weeks.
The sFLT1/PlGF ratio was increased in multiple gestations after 27 weeks' gestation.

Conclusions:

Circulating angiogenic factor levels are altered in multiple gestation pregnancies.
These alterations, particularly the sFLT1/PlGF ratio, may contribute to the increased preeclampsia risk in women with multiple gestations.