Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 7, 2026

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis
06:38

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

Published on: September 12, 2019

Variable antigen expression in hepatoblastomas.

Alan D Ramsay1, Alan W Bates, Susan Williams

  • 1Great Ormond Street Hospital for Children, London WC1N 3JH, UK. a.ramsay@ucl.ac.uk

Applied Immunohistochemistry & Molecular Morphology : AIMM
|January 30, 2008
PubMed
Summary

Hepatoblastoma diagnosis in children is challenging due to variable tumor cell markers. Immunohistochemistry shows hepatoblastomas express diverse antigens, complicating diagnosis and requiring integrated clinical, imaging, and pathology findings.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A fourth pillar for evidence-based medicine: implications for psychiatry - CORRIGENDUM.

The British journal of psychiatry : the journal of mental science·2026
Same author

A fourth pillar for evidence-based medicine: implications for psychiatry.

The British journal of psychiatry : the journal of mental science·2026
Same author

Diagnostic Yield of Post-Mortem Fetal Micro-CT for Central Nervous System Abnormalities.

Prenatal diagnosis·2026
Same author

Germline duplication of MYCN predisposes to childhood embryonal tumours.

EBioMedicine·2026
Same author

Diagnostic Yield of Post-Mortem Fetal Micro-CT for Abdominal and Pelvic Anomalies.

Prenatal diagnosis·2026
Same author

A platform for generating clinical intelligence from routine health data in hospitals: PICTURE.

Future healthcare journal·2026

Area of Science:

  • Pediatric Oncology
  • Surgical Pathology
  • Immunohistochemistry

Background:

  • Hepatoblastoma is a common pediatric liver malignancy, typically diagnosed via needle biopsy.
  • Accurate diagnosis is crucial but challenging due to potential overlap in morphologic features with other childhood tumors.
  • Immunohistochemistry aids in differentiating hepatoblastoma from other small round blue cell tumors.

Purpose of the Study:

  • To investigate the immunophenotypic profile of hepatoblastoma in needle core biopsies.
  • To determine if hepatoblastoma exhibits a distinct diagnostic immunohistochemical phenotype.
  • To assess the utility of standard antibody panels in diagnosing hepatoblastoma.

Main Methods:

  • Analysis of 12 hepatoblastoma needle core biopsies confirmed by resection.

More Related Videos

High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors
19:02

High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors

Published on: March 22, 2011

A Hepatocellular Cancer Patient-Derived Organoid Xenograft Model to Investigate Impact of Liver Regeneration on Tumor Growth
08:15

A Hepatocellular Cancer Patient-Derived Organoid Xenograft Model to Investigate Impact of Liver Regeneration on Tumor Growth

Published on: February 2, 2024

Related Experiment Videos

Last Updated: Jul 7, 2026

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis
06:38

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

Published on: September 12, 2019

High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors
19:02

High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors

Published on: March 22, 2011

A Hepatocellular Cancer Patient-Derived Organoid Xenograft Model to Investigate Impact of Liver Regeneration on Tumor Growth
08:15

A Hepatocellular Cancer Patient-Derived Organoid Xenograft Model to Investigate Impact of Liver Regeneration on Tumor Growth

Published on: February 2, 2024

  • Immunohistochemical staining using antibodies for cytokeratins, alpha-fetoprotein, alpha-1-antitrypsin, MIC-2 (CD99), NCAM (CD56), NB84, desmin, BCL2, vimentin, PGP9.5, and neurone-specific enolase.
  • Evaluation of marker expression in relation to tumor diagnosis and differential diagnosis of childhood malignancies.

    • Main Results:

    • Hepatoblastomas commonly expressed cytokeratins (10/12).
    • Alpha-fetoprotein and alpha-1-antitrypsin staining were positive in 7/12 and 5/12 cases, respectively.
    • Frequent expression of antigens associated with other tumors was observed, including MIC-2 (CD99) in 8/12, NCAM (CD56) in 4/12, and NB84 in 3/12 cases.

    Conclusions:

    • Primitive cells in hepatoblastoma display a variable immunophenotype.
    • Hepatoblastoma can express antigens typically found in other pediatric malignancies, lacking a distinct immunohistochemical profile.
    • Diagnosis of hepatoblastoma necessitates a comprehensive approach combining clinical, imaging, and pathological findings.