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Updated: Jul 7, 2026

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry
Published on: March 24, 2023
Intracellular protease activation in apoptosis and cell-mediated cytotoxicity characterized by cell-permeable
Beverly Z Packard1, Akira Komoriya
1OncoImmunin Inc5., 207A Perry Parkway, Suite 6, Gaithersburg, MD 20877, USA. BPackard@PhiPhiLux.com
Abstract:
Over the past decade the importance of signaling from reporter molecules inside live cells and tissues has been clearly established. Biochemical events related to inflammation, tumor metastasis and proliferation, and viral infectivity and replication are examples of processes being further defined as more molecular tools for live cell measurements become available. Moreover, in addition to quantitating parameters related to physiologic processes, real-time imaging of molecular interactions that compose basic cellular activities are providing insights into understanding disease mechanisms as well as extending clinical efficacy of therapeutic regimens. In this review the use of highly cell-permeable fluorogenic substrates that report protease activities inside live cells is described; applications to defining the molecular events of two cellular processes, i.e., apoptosis and cell-mediated cytotoxicity, are then illustrated.
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