[An improved model of a catheterised human bladder for screening bactericidal agents]

Area of Science:

• Medical Microbiology
• Biomaterials Science
• Urology

Background:

• Catheter encrustation and blockage by crystalline Proteus mirabilis biofilm are persistent issues for long-term catheter users.
• Understanding biofilm formation mechanisms is crucial for developing effective prevention strategies.

Purpose of the Study:

• To evaluate the bactericidal and bacteriostatic efficacy of triclosan-blocking solutions.
• To validate a new physiological bladder model for testing antimicrobial agents.

Main Methods:

• A novel human bladder model using catheterized sterile infusion bags was developed.
• Artificial urine inoculated with Proteus mirabilis was used, with samples collected for pH and microbial counts.
• Catheter encrustation was assessed using scanning electron microscopy.

Main Results:

• Triclosan-blocking solutions maintained free catheter drainage, unlike placebo solutions.
• pH levels remained below 7, and microbial counts were stable.
• Scanning electron microscopy confirmed reduced biofilm formation in triclosan-treated models.

Conclusions:

• Triclosan effectively inhibits Proteus mirabilis growth and biofilm formation in the bladder model.
• The study demonstrates triclosan's ability to prevent pH increase and crystalline biofilm development.
• The developed physiological bladder model is adaptable for testing antimicrobial interventions.