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Updated: Jul 7, 2026

High-Throughput Protein Crystallization via Microdialysis
Published on: March 3, 2023
Dialysis and concentration of protein solutions
S M Andrew1, J A Titus, L Zumstein
1Chester College, Chester, United Kingdom.
Abstract:
Conventional dialysis separates small molecules from large molecules by allowing diffusion of only the small molecules through selectively permeable membranes. Dialysis is usually used to change the salt (small-molecule) composition of a macromolecule-containing solution. This unit describes dialysis of large- and small-volume samples using cellulose membranes with pore sizes designed to exclude molecules above a selected molecular weight. A support protocol describes preparation of membranes for dialysis and discusses issues related to the selection of membranes including commercial kits.
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