Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Micro RT-PCR.

C P Paweletz1, L Charboneau, L A Liotta

  • 1National Cancer Institute/NIH, Bethesda, Maryland, USA.

Current Protocols in Cell Biology
|January 30, 2008
PubMed
Summary
This summary is machine-generated.

This study details protocols for isolating, manipulating, and amplifying RNA from microdissected cells and bulk tissue samples, enabling comprehensive molecular analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultra-deep next-generation sequencing of plasma cell-free DNA in patients with advanced lung cancers: results from the Actionable Genome Consortium.

Annals of oncology : official journal of the European Society for Medical Oncology·2019
Same author

Plasma HPV cell-free DNA monitoring in advanced HPV-associated oropharyngeal cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2018
Same author

Amplicon-based next-generation sequencing of plasma cell-free DNA for detection of driver and resistance mutations in advanced non-small cell lung cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2018
Same author

AMPK inhibition enhances apoptosis in MLL-rearranged pediatric B-acute lymphoblastic leukemia cells.

Leukemia·2012
Same author

Nanoparticle technology: addressing the fundamental roadblocks to protein biomarker discovery.

Current molecular medicine·2010
Same author

Critical dependence of blood-borne biomarker concentrations on the half-lives of their carrier proteins.

Journal of theoretical biology·2008
Same journal

Measuring Mitochondrial Respiration in Previously Frozen Biological Samples.

Current protocols in cell biology·2020
Same journal

Proximity Ligation Assay for Detecting Protein-Protein Interactions and Protein Modifications in Cells and Tissues in Situ.

Current protocols in cell biology·2020
Same journal

Methods for Investigating Corneal Cell Interactions and Extracellular Vesicles In Vitro.

Current protocols in cell biology·2020
Same journal

Multiplexed Proximity Biotinylation Coupled to Mass Spectrometry for Defining Integrin Adhesion Complexes.

Current protocols in cell biology·2020
Same journal

Preparation of Extracellular Matrix Paper and Construction of Multi-Layered 3D Tissue Model.

Current protocols in cell biology·2020
Same journal

Metabolic Analysis at the Nanoscale with Multi-Isotope Imaging Mass Spectrometry (MIMS).

Current protocols in cell biology·2020
See all related articles

Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • Accurate RNA analysis is crucial for understanding cellular function and disease.
  • Microdissection allows for targeted analysis of specific cell populations.
  • Efficient RNA isolation and amplification methods are needed for limited starting material.

Purpose of the Study:

  • To present robust protocols for RNA microisolation, manipulation, and amplification.
  • To provide methods applicable to both microdissected cells and small amounts of bulk tissue.
  • To facilitate downstream molecular analyses using limited biological samples.

Main Methods:

  • Microisolation of cells from tissue.
  • RNA extraction and purification techniques.

Related Experiment Videos

  • RNA amplification strategies for low-input samples.

    • Main Results:

    • Validated protocols for RNA isolation from microdissected cells.
    • Demonstrated successful application of protocols to bulk tissue samples (<10 µg).
    • Ensured high-quality RNA suitable for downstream applications.

    Conclusions:

    • The presented protocols are effective for RNA analysis from scarce biological material.
    • These methods enhance the utility of microdissection in molecular studies.
    • The protocols offer a valuable resource for researchers working with limited tissue samples.