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Media for culture of mammalian cells

J D Sato1, M Kan

  • 1Adirondack Biomedical Research Institute, Lake Placid, New York, USA.

Current Protocols in Cell Biology
|January 30, 2008
PubMed

Keywords:
Cell culture guidelinesSerum-free mediaMammalian physiologySpecialty media formulation

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Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media