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Related Concept Videos

Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
Methods of Sterilization I: Physical Methods01:29

Methods of Sterilization I: Physical Methods

As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Cleaning, Sterilization, and Disinfection01:30

Cleaning, Sterilization, and Disinfection

Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Filtration00:53

Filtration

Filtration is a physical separation process that involves passing a suspension through a porous medium to separate solids from fluids. During filtration, solids collect on the porous medium while liquids, also collectively known as the filtrate, pass through. The filtration medium is selected based on the filtration purpose, quantity, and nature of the precipitate. The general criteria for a suitable filtering medium are that it is inert, mechanically strong, nonabsorbent toward dissolved...
Key Techniques in Microbiology01:19

Key Techniques in Microbiology

Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...

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Related Experiment Video

Updated: Jul 7, 2026

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria
09:56

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Published on: October 13, 2021

Sterilization and filtration.

R J Coté1

  • 1Becton Dickinson Microbiology Systems, Sparks, Maryland, USA.

Current Protocols in Cell Biology
|January 30, 2008
PubMed
Summary
This summary is machine-generated.

Maintaining sterility in tissue culture is crucial. This guide details methods for sterilizing liquids and dry goods, plus filtration techniques, to prevent microbial contamination and ensure successful cultures.

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Last Updated: Jul 7, 2026

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Area of Science:

  • Microbiology
  • Cell Biology
  • Biotechnology

Background:

  • Sterility is paramount in tissue culture to prevent microbial contamination.
  • Contamination can lead to experimental failure and loss of valuable cell lines.
  • Standard sterilization protocols are essential for reliable tissue culture work.

Purpose of the Study:

  • To outline effective methods for achieving and maintaining sterility in tissue culture.
  • To describe techniques for sterilizing both liquid and dry materials used in tissue culture.
  • To explain the importance and methods of liquid filtration for contamination prevention.

Main Methods:

  • Discusses autoclaving and dry heat sterilization for dry goods.
  • Details aseptic techniques for handling sterile liquids.
  • Explains filtration methods using various pore sizes for liquid sterilization.

Main Results:

  • Effective sterilization of common tissue culture media and equipment.
  • Demonstration of filtration efficacy in removing microbial contaminants.
  • Prevention of bacterial and fungal contamination in cultures.

Conclusions:

  • Proper sterilization and filtration are critical for successful tissue culture.
  • Adherence to described methods ensures a sterile working environment.
  • Minimizing contamination enhances the reliability and reproducibility of tissue culture experiments.