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Methods for Controlling Microbial Growth

Methods for Controlling Microbial Growth

Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...

Key Techniques in Microbiology

Key Techniques in Microbiology

Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...

Bioreactor Controls-I

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...

Scale-Up Processes

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...

Methods to Assess Microbial Populations

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Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

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Related Experiment Video

Updated: Jul 7, 2026

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Published on: July 7, 2023

Assessing and controlling microbial contamination in cell cultures.

¹Becton Dickinson Microbiology Systems, Sparks, Maryland, USA.

Current Protocols in Cell Biology

|January 30, 2008

Summary

Detect and manage microbial contamination in cell cultures. This guide covers identifying bacterial, fungal, and mycoplasma contaminants and offers strategies for remediation to ensure culture integrity.

Area of Science:

Microbiology
Cell Culture Technology

Background:

Microbial contamination poses a significant threat to cell culture experiments.
Bacterial, fungal, and mycoplasma contaminants can compromise experimental results and viability.

Purpose of the Study:

To provide a comprehensive guide for detecting and recognizing microbial contamination in cell cultures.
To outline methods for verifying the presence of mycoplasma.
To describe strategies for managing and eliminating contamination when necessary.

Main Methods:

Visual inspection of cultures for signs of contamination.
Microscopic examination for bacterial and fungal identification.
Specific assays for mycoplasma detection and verification.

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Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

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The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

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Last Updated: Jul 7, 2026

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Published on: July 7, 2023

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

Published on: June 16, 2022

Main Results:

Effective methods for identifying common microbial contaminants are presented.
Protocols for confirming mycoplasma presence are detailed.
Actionable strategies for dealing with contaminated cultures are described.

Conclusions:

Prompt and accurate detection of microbial contamination is crucial for maintaining cell culture integrity.
Implementing verification methods ensures reliable identification of contaminants.
Proactive management strategies are essential for successful cell culture work.