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Purification of vitronectin

S K Akiyama1

  • 1Chapel Hill, North Carolina, USA.

Current Protocols in Cell Biology
|January 30, 2008
PubMed

Abstract:

This unit describes the purification of extracellular vitronectin from plasma by a series of affinity chromatography steps. First plasma is depleted of fibronectin and other heparin-binding proteins; the sample is then treated with urea to activate the heparin-binding activity of vitronectin. The resulting vitronectin is approximately 98% pure.

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