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Related Experiment Videos

Invasion assays.

H K Kleinman1, K Jacob

  • 1National Institute of Dental Research/NIH, Bethesda, Maryland, USA.

Current Protocols in Cell Biology
|January 30, 2008
PubMed
Summary
This summary is machine-generated.

Tumor cells invade by degrading the basement membrane. Matrigel-coated Boyden chambers provide a reliable in vitro model to study this crucial step in cancer metastasis.

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Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • The basement membrane is a critical barrier preventing tumor cell invasion and metastasis.
  • Tumor cells degrade the basement membrane using proteases to invade surrounding tissues.
  • Understanding tumor cell invasion mechanisms is vital for developing anti-metastatic therapies.

Purpose of the Study:

  • To present a reliable and reproducible in vitro model for studying tumor cell invasion through the basement membrane.
  • To investigate the process of tumor cell migration and invasion across a Matrigel-coated membrane.

Main Methods:

  • Utilizing a modified Boyden chamber assay with a Matrigel-coated porous membrane.
  • Placing tumor cells in the upper chamber and a chemoattractant in the lower chamber.

Related Experiment Videos

  • Quantifying tumor cell migration and invasion by counting cells on the lower membrane surface after incubation.

    • Main Results:

    • The Matrigel-coated Boyden chamber assay effectively models in vivo tumor cell invasion.
    • The assay allows for reliable and reproducible measurement of tumor cell migration across the basement membrane.
    • This model facilitates the study of factors influencing tumor cell invasion.

    Conclusions:

    • Matrigel-based in vitro models are superior for studying tumor cell invasion compared to other methods.
    • The presented assay provides a robust platform for evaluating anti-invasion strategies.
    • Further research using this model can elucidate key mechanisms of cancer metastasis.