Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

Antibody Structure

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Low risk management intervention: Limited impact of remedial tillage on net ecosystem carbon balance at a commercial Miscanthus plantation.

Global change biology. Bioenergy·2024

Same author

Overriding water table control on managed peatland greenhouse gas emissions.

Nature·2021

Same author

Sphingosine kinase 1 overexpression induces MFN2 fragmentation and alters mitochondrial matrix Ca<sup>2+</sup> handling in HeLa cells.

Biochimica et biophysica acta. Molecular cell research·2019

Same author

Evaluation of the safety and adjuvant effect of a detoxified listeriolysin O mutant on the humoral response to dengue virus antigens.

Clinical and experimental immunology·2016

Same author

dcc orchestrates the development of the prefrontal cortex during adolescence and is altered in psychiatric patients.

Translational psychiatry·2013

Same author

Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis.

Nucleic acids research·2012

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Production of polyclonal antisera.

H M Cooper¹, Y Paterson

¹Ludwig Institute of Cancer Research, Melbourne, Australia.

Current Protocols in Cell Biology

|January 30, 2008

Summary

This summary is machine-generated.

This study details the production of polyclonal antibodies in various species, enhancing sensitivity in assays like ELISAs and immunoprecipitation. Protocols cover serum preparation and generating antipeptide antibodies for diverse research applications.

More Related Videos

Generation of Monoclonal Antibodies Against Natural Products

Generation of Monoclonal Antibodies Against Natural Products

Published on: April 6, 2019

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Related Experiment Videos

Last Updated: Jul 7, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Generation of Monoclonal Antibodies Against Natural Products

Generation of Monoclonal Antibodies Against Natural Products

Published on: April 6, 2019

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Area of Science:

Immunology
Biotechnology

Background:

Polyclonal antibody preparations feature multiple antibody molecules targeting diverse epitopes on an antigen.
High-quality polyclonal antisera enhance assay sensitivity through multi-molecule binding, crucial for techniques like immunoprecipitation, immunoblotting, and ELISAs.

Purpose of the Study:

To describe protocols for producing polyclonal antisera specific for protein antigens in rabbits, rats, mice, and hamsters.
To present a method for preparing serum from blood.
To outline the production of polyclonal antipeptide antisera.

Main Methods:

Immunization of rabbits, rats, mice, and hamsters with protein antigens using complete Freund's adjuvant (CFA) or other adjuvants.
Serum preparation from blood samples.
Substitution of carrier-conjugated peptides for purified protein antigens to generate antipeptide antibodies.

Main Results:

Successful generation of polyclonal antisera in multiple species.
Demonstrated utility of polyclonal antibodies in enhancing immunoassay sensitivity.
Established methods for both polyclonal antipeptide and protein antigen antisera production.

Conclusions:

Polyclonal antibody production protocols are presented for various species and antigen types.
The described methods facilitate sensitive detection in immunological assays.
These protocols support diverse applications in immunological research and diagnostics.